Invitrogen nupage manual

Band quality with Novex Tris-Glycine gels. Protein ladders, purified proteins, and E. coli lysate were loaded on an Invitrogen Novex Tris-Glycine Gel, 4–20% gradient (A) and a supplier 4–20% gradient gel (B).Straighter lanes with better lysate protein band sharpness and resolution are observed on gel (A).Lanes 1, 5, 5 µL Thermo Scientific PageRuler Unstained Protein . User Manual Corporate Headquarters Invitrogen Corporation Faraday Avenue Carlsbad, CA T: 1 7F: 1 7E: www.doorway.rue@www.doorway.ru For country-specific contact information visit our web site at www.doorway.ru Novex® Semi-Dry Blotter For semi-dry electroblotting of proteins from polyacrylamide and agarose gels. Protein separation comparing NuPAGE Bis-Tris Gel and traditional tris-glycine gel. The samples listed below were run on (A) a NuPAGE 4–12% Bis-Tris Gel in Invitrogen NuPAGE MES SDS Running Buffer or (B) another manufacturer’s 4–20% tris-glycine gel. Note the high resolution of the sample bands in the NuPAGE protein gel.

NuPAGE Sample Reducing Agent (10X) is used to reduce protein samples for protein gel electrophoresis. It contains mM dithiothreitol (DTT) for a 10X concentration in a stabilized liquid form. See all available buffers and reagents available for SDS-PAGE. For Research Use Only. *NuPAGE® Transfer Buffer with 10% methanol provides optimal transfer of a single gel in the blot module. If you are transferring 2 gels in the blot module, increase the methanol content to 20% to ensure efficient transfer of both gels. Refer to page 30 for a recipe of the NuPAGE® Transfer Buffer, if you are preparing your own transfer buffer. NuPAGE™ Tris-Acetate 7%, 3–8% 36 to kDa 6 months 60 min Separation of larger proteins. Novex™ Tricine 10%, 16% 2 to kDa 1–2 months 90 min Separation of small proteins and peptides. NativePAGE™ Bis-Tris 3–12%, 4–16% 15 to 10, kDa 12 months 90– min Separation of native proteins.

Jun rapid protocol is optimized for Invitrogen NuPAGE® gels, but will give comparable results with most types of minigels. The. NuPAGE. ® electrophoresis system. Rev. date: 29 October Manual part no. The rapid protocol is optimized for Invitrogen NuPAGE® gels, and can be. Instructions for electrophoresis using the XCell SureLock® Mini-Cell are refer to the NuPAGE® Technical Guide available at www.doorway.ru